The solution and place it in the spectrophotometer.Ĭ. To Determine the maximum wavelength (~460nm) : Repeat for the remaining calibration solutions, recording absorbances to three decimal places in a data table in your lab notebook. Rinse in the cuvette with solution #2, wipe it off, insert it into the instrument, and read the absorbance at the maximum wavelength. Spectrophotometer make sure to align the cuvette so that the clear sides areįacing the light source of the spectrometer.
#LOGGER PRO ABSORBANCE FULL#
Prepare a blank by filling an empty cuvette ¾ full Make all five solutions before proceeding to Part 1b of the procedure. Rinse the volumetric flask thoroughly with distilled water before preparing the next solution. Transfer each solution to a clean, dry flask labeled with the calibration solution number. Stopper the flask securely and mix completely by inverting and rotating the flask several times. Add the last mL with a dropper in order to avoid filling past the mark. Increase the solution volume to precisely 25.00 mL by adding 0.1 M HNO 3 until the flask is filled to the mark. Use a repipetter for the 0.200M Fe(NO 3) 3 and a graduated pipet for the 2.00 x 10 -3 M KSCN. Prepare each calibration solution by pipetting the amounts of 0.200M Fe(NO 3 ) 3 and 2.00 x 10 - 3 M KSCN shown in Table 1 into a clean 25-mL volumetric flask. Enter data and results in your lab notebook as you proceed. In your lab notebook, set up a data table in the format shown in Part 1 of the Report Form (Table 1). Read instructions and reagent labels carefully. Obtaining Calibration Data for FeSCN 2+ SolutionsĬAUTION: The Fe(NO 3 ) 3 reagents for Parts 1 and 2 are different: 0.200 M in Part 1, and 0.00200 M in Part 2. Carry out all other work and reporting individually. You will hand in the yellow copies of your lab work with the Report Form. Insert the cuvette into the colorimeter as well and use the line of best fit to determine its average concentration level.Be sure to read and study the BACKGROUND material, along with these procedures, before coming to lab.Īs you work, keep full, legible records of your work, data, and observations in your Laboratory Notebook. Insert this cuvette into the colorimeter and use the line of best fit to determine its concentration.ġ1) Add the 3rd solution with an unknown concentration to a cuvette. Insert cuvette into the colorimeter and use the line of best fit to determine its concentration.ġ0) Add the 2nd solution with one of the unknown concentrations to a cuvette. Insert cuvette into the colorimeter and record the absorbance.Ĩ) Set a line of best fit that runs through the middle of your data to have an average of the data plotted.ĩ) Add the 1st solution with one of the unknown concentrations to a cuvette. Insert cuvette into the colorimeter and record the absorbance.ħ) Add the pure 0.4 M nickel nitrate solution to a cuvette. Add 2 mL of distilled water to the test tube. Insert the cuvette into the colorimeter and record the absorbance.Ħ) Add 8 mL of the nickel nitrate solution to a test tube. After that add 4 mL of distilled water to the test tube and stir thoroughly.
Insert cuvette into the colorimeter and record the absorbance.Ĥ) Next, add 4 mL of the nickel nitrate solution to a test tube, this time add 6 mL of distilled water to the test tube and of course stir thorough it properly. Pour this concentrated solution into a cuvette as well and insert the cuvette into the colorimeter and record the absorbance.ĥ) Continuing on, add yet another 6 mL of nickel nitrate solution to a test tube. Stir thoroughly to make the solution concentrated. To reduce add 8 mL of distilled water to the test tube.
Kandace-We wont see anything!)<- See i was here for that conversation!!! :)Ģ) Set the colorimeter to red light not green!ģ) To begin with add 2 mL of nickel nitrate solution to a test tube. Ludwig-Why dont we want it to be on green if the solution is green? 1.) Goggle time! Put those goggles on people, Caution when using NiSO4 solutions.Ģ.) Add distilled water to a cuvette and insert it in the colorimeter, then set the colorimeter to no light and calibrate the unit.
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